Components

Respiratory Viral Screen - (Catalog No. 5007). One (1) 10 ml dropper vial containing monoclonal antibody against: Adenovirus, Influenza A, Influenza B, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, and Respiratory Syncytial Virus.Normal Mouse Antibody - (Catalog No. 5014). One (1) 10 ml dropper vial containing Normal Mouse Antibody to be used as a negative control.Anti-Mouse IgG/FITC Conjugate - (Catalog No. 5008). One (1) 10 ml dropper vial containing FITC Labeled Goat Anti-Mouse IgG Antibody.PBS - (Catalog No. 5087). One (1) packet of Phosphate Buffered Saline Salts yields 1 liter when dissolved in distilled water. Store in a clean closed container at room temperature.Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037) One (1) 10 ml vial containing Polyoxyethylene Sorbitan Monolaurate (Tween 20) and Sodium Azide (NaN3) concentrate to be diluted 1:100 in PBS.Mounting Fluid - (Catalog No. 5013). One (1) 10 ml dropper vial containing Tris Buffer, Glycerin, Fluorescence Enhancer, and Sodium Azide as preservative. Store at room temperature.

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Storage and Stability

When stored at 2-8°C, the Respiratory Viral Screen is stable up to the expiration date printed on the kit label.

During incubation, slides should be protected from light and kept in a humid chamber at the recommended temperature.

Do not freeze or expose to elevated temperatures.

A marked decrease in fluorescence may indicate conjugate or antibody deterioration. A positive control should be tested with each specimen to ensure proper functioning of these reagents and proper staining procedure. If after appropriate analysis there is a decrease in staining intensity, discontinue use of the reagents.

Warnings and Precautions:

⋅ Sodium Azide (present in the conjugate, respiratory viral screen, PBS solution, and mounting fluid) can form potentially explosive metal azides with plumbing. Flush with large volumes of water to prevent azide build up.

⋅ Pooling or diluting conjugate or respiratory viral screen antibody may cause erroneous results.

⋅ Do not use reagents after expiration.

⋅ Avoid leaving reagents above 2-8°C for extended periods.

⋅ Do not allow slides to dry at any time during the staining procedure.

⋅ Handle all specimens and materials as potentially infectious. Decontaminate with 0.05% Sodium Hypochlorite (a 1:100 dilution of household bleach).

⋅ Do not expose reagents to bright light during storage or incubation.

⋅ Avoid contact with Evans Blue (present in the anti-mouse IgG/FITC conjugate) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks, or flames. Avoid breathing vapor. Use adequate ventilation.

⋅ Do not mouth pipette reagents.

⋅ Do not substitute reagents from other manufacturers.

⋅ Alteration of protocol provided may cause erroneous results.

⋅ When staining multiple samples on a slide, avoid cross contamination between samples.

⋅ Normal mouse antibody should be tested with each cell culture isolate. Fluorescence indicates a non-specific reaction and the test is considered invalid.